![]() A higher erythrocyte count at week 2 in male rats fed 10% carnauba wax was noticed, but no other statistical significant differences were recorded on haematological findings in rats fed diet containing 10% carnauba wax at 2, 6 or 13 weeks. Results showed a significant increase in mean food consumption in male and female rats given 10% carnauba wax or 10% cellulose powder, without statistically significant differences in body weights compared to controls. Histopathological examinations were done on half of the tissue sections conserved from the control rats and tissues from those given 10% carnauba wax or 10% cellulose powder. Hematological, serum biochemistry, urinalysis, organ weights, and gross examinations were recorded at autopsy. Food consumption and body weights were recorded. Additional groups of five rats of each sex were fed diets containing 0, 5, 10% carnauba wax or 10% cellulose powder for 2 and 6 weeks. The rats fed diets containing cellulose powder acted as a control group for possible effects due to the replacement of a significant proportion of the diet by a non-nutrient test material. Groups of 15 male and 15 female Wistar rats, approximately 5 weeks of age at the beginning of the study, were fed diets containing 0, 1, 5 or 10% carnauba wax, or 10% cellulose powder for 13 weeks corresponding to 0, 800, 42 mg/kg body weight per day for males and 0, 900, 4600 and 10 200 mg/kg body weight per day for females 35). The Scientific Committee for Food noted that a use level of 1200 mg/kg of food would yield a worst-case intake estimate of 48 mg/person/day which equates to 4.8 mg/kg body weight day for a 10 kg child, or less for an adult, and is within the ADI of 0-7 mg/kg body weight set by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) in 1993 28). The Scientific Committee for Food did not object the use of carnauba wax as a glazing agent at higher levels in products requiring it. In an addendum to the latter opinion, Scientific Committee for Food considered a new request for use levels of carnauba wax of 500 mg/kg in confectionery (hard and soft sugar coated centers) and 1200 mg/kg in chewing gum as a glazing agent 27). The Scientific Committee for Food decided to withdraw the temporary acceptance status of carnauba wax and accepted its use as a glazing agent up to a maximum use level of 200 mg/kg of food 26). ![]() In 2001 the Scientific Committee for Food reviewed new genotoxicity assay and supplementary information on usage levels of carnauba wax as a glazing agent submitted following its request in 1997 25). The European Food Safety Authority Panel noted that hydrocarbons would only constitute between 0.3 and 1% of the total composition of carnauba wax 5). Cinnamic aliphatic esters are described as consisting of p-methoxycinnamic acid and dihydric alcohols with even-numbered carbon chains from C24 to C34 4). Αlpha-hydroxyl esters are described as consisting of straightchain hydroxyl acids with even-numbered carbon chains from C22 to C28, straight-chain acids with even-numbered carbon chains form C24 to C28, straight-chain monohydric alcohols with even numbered carbon chains from C24 to C34 and dihydric alcohols with even-numbered carbon chains from C24 to C34. Aliphatic esters are described by Joint FAO/WHO Expert Committee on Food Additives (JECFA) as consisting of straight-chain acids with even-numbered carbon chains from C24 to C28 and straight-chain alcohols with even numbered carbon chains from C30 to C34. These properties also make Carnauba Wax an ideal depilatory agent for temporarily removing unwanted body hair.Ĭarnauba wax also contains free acids, free alcohols, hydrocarbons and resins 3). This easy spreadability is what makes Carnauba Wax ideal for incorporation into makeup, as it keeps the form elastic rather than stiff and this yield gives makeup easy application as well as adjustability. This in turn allows the products to retain their solid structures when they glide smoothly across the skin. ![]() When applied to formulations for stick products, such as deodorants, lipsticks, or stick foundations, the combination of Carnauba Wax with any of these other waxes helps to boost the density of the lipids. This helps create products with lasting creamy consistencies. In any of these combinations, Carnauba Wax boosts the binding properties of ingredients in emulsions, allowing liquids to bind easily with chemical ingredients, particularly oils. Carnauba Wax combines well with several other waxes from plant, animal, and mineral sources, as well as with various resins, whether natural or synthetic.
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